β-actin Rabbit pAb
ENT-A1143
Description
| REF | ENT-A1143 |
|---|---|
| Category | Antibody Polyclonal |
| Description | β-actin Rabbit pAb |
| Source | Rabbit |
| Applications | WB, IHC, IF |
| Reactivity | Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus |
| Reactivity | Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus |
| Dilution | WB 1:3,000 IHC 1:200 IF 1:200 |
| Immunogen | Recombinant Protein |
| Storage Stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/mL |
| Observed Band KD | 43kDa |
| Human Gene ID | 60 |
| Human Swiss Prot Nº | P60709 |
| Subcellular Location | Cytoplasm,Cytoskeleton |
Other Name: actin antibody, b-actin antibody, beta-actin antibody
Background: β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.