VSV-G-Tag Mouse mAb
ENT-A1391
Description
| Ref | ENT-A1391 |
|---|---|
| Category | Antibody Monoclonal |
| Alternative Name | |
| Application | WB |
| Source | Mouse |
| Reactivity | |
| Dilution | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Immunogen | Synthetic Peptide |
| Storage Stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Concentration | 1mg/mL |
| Observed Band(KD) | |
| Human Gene ID | |
| Human Swiss Prot No | |
| Subcellular Location |
Background: Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation.