Topo IIα (phospho Ser1525) rabbit pAb
ENT-A7149
Description
| REF | ENT-A7149 |
|---|---|
| Category | Antibody Polyclonal |
| Description | Topo IIα (phospho Ser1525) rabbit pAb |
| Source | Rabbit |
| Applications | WB;IHC;IF;ELISA |
| Reactivity | Human;Mouse;Rat |
| Reactivity | Human;Mouse;Rat |
| Dilution | Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TOP2A around the phosphorylation site of Ser1525. AA range:1482-1531 |
| Storage Stability | -20°C/1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band KD | 190kD |
| Human Gene ID | 7153 |
| Human Swiss Prot Nº | P11388 |
| Subcellular Location | Cytoplasm . Nucleus, nucleoplasm . Nucleus . Nucleus, nucleolus . |
Other Name: TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme
Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla