Rock-1 rabbit pAb
ENT-A3641
Description
| REF | ENT-A3641 |
|---|---|
| Category | Antibody Polyclonal |
| Description | Rock-1 rabbit pAb |
| Source | Rabbit |
| Applications | IF;WB;IHC;ELISA |
| Reactivity | Human;Mouse;Rat;Monkey |
| Reactivity | Human;Mouse;Rat;Monkey |
| Dilution | IF: 1:50-200 Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunocytochemistry: 1/200 – 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Rock-1. AA range:262-311 |
| Storage Stability | -20°C/1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band KD | 158kD |
| Human Gene ID | 6093 |
| Human Swiss Prot Nº | Q13464 |
| Subcellular Location | Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus membrane ; Peripheral membrane protein . Cell projection, bleb . Cytoplasm, cytoskeleton . Cell membrane . Cell projection, lamellipodium . Cell projection, ruffle . A small proportion is associated with Golgi membranes (PubMed:12773565). Associated with the mother centriole and an intercentriolar linker (By similarity). Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers (By similarity). Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). . |
Other Name: ROCK1; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
Background: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015],