Recombinant E.coli Trp synthase (N-6His)
Recombinant E.coli Tryptophan Synthase is produced by our E.coli expression system and the target gene encoding Met1-Ser268&Thr2-Ile397 is expressed with a 6His tag at the N-terminus.
Description
| Reference | ESCIT234 |
|---|---|
| Size | 50ug |
| Molecular Weight | 28.7&43.8 KDa |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Biological Activity |
Other names: Tryptophan synthetase; Tryptophan synthase
Redissolve:
Storage: Store at ?-70°C, stable for 6 months after receipt.
Store at ?-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
Background: Tryptophan synthase is a multienzyme ?2?2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two stESCIs in the synthesis of L-tryptophan (L-Trp). The ?-subunit catalyzes cleavage of 3-indole-d-glycerol 3?-phosphate (IGP) to give indole and D-glyceraldehyde 3?-phosphate (G3P). Indole is then transferred through a 25-Å hydrophobic tunnel to the ?-subunit. The ?2 subunit contains pyridoxal 5′-phosphate and catalyzes several pyridoxal 5′-phosphate-dESCIendent reactions, including/3-elimination reactions 6 and a thiol-dESCIendent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.