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MBP-Tag Mouse mAb

MBP-Tag Mouse mAb

ENT-A1392

Description

Ref ENT-A1392
Category Antibody Monoclonal
Alternative Name
Application WB
Source Mouse
Reactivity
Dilution WB 1:5,000
Immunogen Recombinant Protein
Storage Stability PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality Monoclonal
Isotype IgG1
Concentration 1mg/mL
Observed Band(KD)
Human Gene ID
Human Swiss Prot No
Subcellular Location

Background: Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest.