Human TRH(Thyrotropin Releasing Hormone) ELISA Kit
Human TRH(Thyrotropin Releasing Hormone) ELISA Kit
Description
| Reference | ENT-E14034 |
|---|---|
| Category | Human O-Z |
| Size | 96T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 2.7 pg/mL |
| Standard | 500 pg/mL |
| Detection Range | 7.82-500 pg/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 2h |
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Research Area: Endocrinology;Neuro science;Hepatology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor