Human SP-D(Pulmonary surfactant-associated protein D) Microsample ELISA Kit
Human SP-D(Pulmonary surfactant-associated protein D) Microsample ELISA Kit
Description
| Reference | ENT-E33346 |
|---|---|
| Category | Human O-Z |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 0.51 ng/mL |
| Standard | 80 ng/mL |
| Detection Range | 1.25-80 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Infection immunity;Pulmonology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SP-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SP-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: SFTPD; SPD; SFTP 4;