Human sIL-6R(SolubleInterleukin 6 Receptor) Microsample ELISA Kit
Human sIL-6R(SolubleInterleukin 6 Receptor) Microsample ELISA Kit
Description
| Reference | ENT-E12796 |
|---|---|
| Category | Human O-Z |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 0.78 ng/mL |
| Standard | 120 ng/mL |
| Detection Range | 1.88-120 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates, urine and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor