Human sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
Human sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
Description
| Reference | ENT-E8656 |
|---|---|
| Category | Human O-Z |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 12.9 ng/mL |
| Standard | 2000 ng/mL |
| Detection Range | 31.25-2000 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9