Human PPAR-?(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
Human PPAR-?(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
Description
| Reference | ENT-E10850 |
|---|---|
| Category | Human O-Z |
| Size | 48T |
| Assay Type | Sandwich |
| Sensitivity | 0.057 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-?. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPAR-?. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPAR-?, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPAR-? in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma