Human POMC(Proopiomelanocortin) ELISA Kit
Human POMC(Proopiomelanocortin) ELISA Kit
Description
| Reference | ENT-E11583 |
|---|---|
| Category | Human O-Z |
| Size | 96T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 44 pg/mL |
| Standard | 10000 pg/mL |
| Detection Range | 156.25-10000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Lenght | 2h |
Research Area: Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human POMC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POMC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POMC in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro Opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone