Human p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
Human p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
Description
| Reference | ENT-E9366 |
|---|---|
| Category | Human O-Z |
| Size | 96T |
| Assay Type | Sandwich |
| Sensitivity | 0.121 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1