Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Description
| Reference | ENT-E9704 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 15 pg/mL |
| Standard | 2500 pg/mL |
| Detection Range | 39.07-2500 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Endocrinology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb