Human NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) Microsample ELISA Kit
Human NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) Microsample ELISA Kit
Description
| Reference | ENT-E5954 |
|---|---|
| Category | Human F-N |
| Size | 48T |
| Assay Type | Sandwich |
| Sensitivity | 0.32 ng/mL |
| Standard | 50 ng/mL |
| Detection Range | 0.79-50 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Endocrinology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: NT-Pro-ANP; N-ANP