Human NF-?Bp105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit
Human NF-?Bp105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit
Description
| Reference | ENT-E12735 |
|---|---|
| Category | Human F-N |
| Size | 96T |
| Assay Type | Sandwich |
| Sensitivity | 0.058 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
PDF File:
Research Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NF-?Bp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NF-?Bp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NF-?Bp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NF-?Bp105 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B