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Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit

Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit

Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit

Description

Reference ENT-E3839
Category Human F-N
Size 48T
Assay Type Competitive Inhibition
Sensitivity 0.28 ng/mL
Standard 50 ng/mL
Detection Range 0.79-50 ng/mL
Sample Type cell lysates and other biological fluids
Assay Lenght 2h

PDF File: 

Research Area: Developmental science;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: RPS12; MRPS12; MRPS34; PSMD7; HTR1B