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Human MEP1a(Meprin A Alpha) ELISA Kit

Human MEP1a(Meprin A Alpha) ELISA Kit

Human MEP1a(Meprin A Alpha) ELISA Kit

Description

Reference ENT-E23472
Category Human F-N
Size 96T
Assay Type Competitive Inhibition
Sensitivity 17 pg/mL
Standard 4000 pg/mL
Detection Range 62.5-4000 pg/mL
Sample Type Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Lenght 2h

PDF File:  https://enteksci.com/wp-content/uploads/2025/10/ENT-E23472.pdf

Research Area: Metabolic pathway;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MEP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase