Human MDH1(Malate Dehydrogenase 1) ELISA Kit
Human MDH1(Malate Dehydrogenase 1) ELISA Kit
Description
| Reference | ENT-E19481 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 27 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MDH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MDH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MDH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MDH1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: MDHA; Malate Dehydrogenase 1,NAD(Soluble); Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase