Human Lptn/LTN/XCL1 (Lymphotactin) Microsample ELISA Kit
Human Lptn/LTN/XCL1 (Lymphotactin) Microsample ELISA Kit
Description
| Reference | ENT-E4478 |
|---|---|
| Category | Human F-N |
| Size | 48T |
| Assay Type | Sandwich |
| Sensitivity | 0.12 ng/mL |
| Standard | 25 ng/mL |
| Detection Range | 0.39-25 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Assay Lenght | 3.5h |
Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1