Human LHb(Luteinizing Hormone Beta Polypeptide) ELISA Kit
Human LHb(Luteinizing Hormone Beta Polypeptide) ELISA Kit
Description
| Reference | ENT-E23509 |
|---|---|
| Category | Human F-N |
| Size | 48T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 88.5 pg/mL |
| Standard | 20000 pg/mL |
| Detection Range | 312.5-20000 pg/mL |
| Sample Type | Serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 2h |
Research Area: Reproductive science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human LHb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LHb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LHb in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: LH-B; CGB4; LSH-B; hLHB; Lutropin Subunit Beta; Interstitial Cell Stimulating Hormone,Beta Chain