Human LAL(Lysosomal Acid Lipase) ELISA Kit
Human LAL(Lysosomal Acid Lipase) ELISA Kit
Description
| Reference | ENT-E3145 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 27.1 pg/mL |
| Standard | 4000 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAL in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: LIPA; CESD; Lipase A,Lysosomal Acid,Cholesterol Esterase(Wolman Disease); Acid Cholesteryl Ester Hydrolase; Lysosomal acid lipase/cholesteryl ester hydrolase