Human KLK1(Kallikrein 1) ELISA Kit
Human KLK1(Kallikrein 1) ELISA Kit
Description
| Reference | ENT-E6813 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.12 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Lenght | 2h |
Research Area: Enzyme & Kinase;Tumor immunity;Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein