Human IFNa/bR2(Interferon Alpha/Beta Receptor 2) ELISA Kit
Human IFNa/bR2(Interferon Alpha/Beta Receptor 2) ELISA Kit
Description
| Reference | ENT-E17272 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 0.25 ng/mL |
| Standard | 40 ng/mL |
| Detection Range | 0.63-40 ng/mL |
| Sample Type | Serum, plasma, urine and other biological fluids. |
| Assay Lenght | 3.5h |
Research Area: Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNa/bR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNa/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNa/bR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: IFN?R2; IFN-alpha-REC; IFN-R; IFN?BR; IFN?RB; Interferon(Alpha,Beta And Omega)Receptor Beta Chain; Interferon alpha binding protein; Type I interferon receptor 2