Human I-PTH(IntactParathormone) ELISA Kit
Human I-PTH(IntactParathormone) ELISA Kit
Description
| Reference | ENT-E13375 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 4.81 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone