Human HVD3(25-Hydroxyvitamin D3) ELISA Kit
Human HVD3(25-Hydroxyvitamin D3) ELISA Kit
Description
| Reference | ENT-E33546 |
|---|---|
| Category | Human F-N |
| Size | 96T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.88 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 2h |
PDF File: https://enteksci.com/wp-content/uploads/2025/10/ENT-E33546.pdf
Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D