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Human HBb(Hemoglobin Beta) ELISA Kit

Human HBb(Hemoglobin Beta) ELISA Kit

Human HBb(Hemoglobin Beta) ELISA Kit

Description

Reference ENT-E19135
Category Human F-N
Size 96T
Assay Type Competitive Inhibition
Sensitivity 2.82 pg/mL
Standard 500 pg/mL
Detection Range 7.82-500 pg/mL
Sample Type serum, plasma and erythrocyte lysates
Assay Lenght 2h

PDF File:  https://enteksci.com/wp-content/uploads/2025/10/ENT-E19135.pdf

Research Area: Hematology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBb in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: HB-B; HBD; CD113t-C; Beta Globin; Spinorphin