Human GA(Glycated Albumin) ELISA Kit
Human GA(Glycated Albumin) ELISA Kit
Description
| Reference | ENT-E32992 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 5.03 pmol/mL |
| Standard | 1000 pmol/mL |
| Detection Range | 15.63-1000 pmol/mL |
| Sample Type | serum, plasma |
| Assay Lenght | 2h |
Research Area: diabetic nephropathy
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: Glycated Albumin,GA