Human CRH(Corticotropin Releasing Hormone) ELISA Kit
Human CRH(Corticotropin Releasing Hormone) ELISA Kit
Description
| Reference | ENT-E5969 |
|---|---|
| Category | Human A-E |
| Size | 48T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.13 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 2h |
Research Area: Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: CRF; Corticotropin Releasing Factor; Corticoliberin