Description

PDF File: 

Research Area: Signal transduction;Metabolic pathway;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human cGMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human cGMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: c-GMP; 3′,5′-Cyclic GMP; Cyclic 3′,5′-GMP; Guanosine 3′,5′-Cyclic Phosphate