Human CA 27-29(cancer antigen 27-29) ELISA Kit
Human CA 27-29(cancer antigen 27-29) ELISA Kit
Description
| Reference | ENT-E33475 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 0.8 U/mL |
| Standard | 100 U/mL |
| Detection Range | 1.57-100 U/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Assay Lenght | 3.5h |
Research Area: Cancer
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: CA 27-29