Human BAFF/CD257(B-Cell Activating Factor) ELISA Kit
Human BAFF/CD257(B-Cell Activating Factor) ELISA Kit
Description
| Reference | ENT-E12463 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 28 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF