Human B2-glycoprotein 1 (?2-GP1) antibody lgG ELISA Kit
Human B2-glycoprotein 1 (?2-GP1) antibody lgG ELISA Kit
Description
| Reference | ENT-E4108 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ?2-GP1 lgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ?2-GP1 lgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ?2-GP1 lgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ?2-GP1 lgG in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: APOH; BG; B2G1; B2GP1