Human AP1M1(AP-1 Complex Subunit Mu-1) ELISA Kit
Human AP1M1(AP-1 Complex Subunit Mu-1) ELISA Kit
Description
| Reference | ENT-E1671 |
|---|---|
| Category | Human A-E |
| Size | 96T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.11 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 2h |
Research Area: Metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP1M1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP1M1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP1M1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: AP47; CLAPM2; CLTNM; MU-1A; mu1A; Adaptor Related Protein Complex 1 Subunit Mu 1; AP-mu chain family member mu1A