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Human AEA(Anandamide) ELISA Kit

Human AEA(Anandamide) ELISA Kit

Human AEA(Anandamide) ELISA Kit

Description

Reference ENT-E33291
Category Human A-E
Size 96T
Assay Type Competitive Inhibition
Sensitivity 0.9 ng/mL
Standard 200 ng/mL
Detection Range 3.13-200 ng/mL
Sample Type serum, plasma and other biological fluids
Assay Lenght 2h

PDF File:  https://enteksci.com/wp-content/uploads/2025/10/ENT-E33291.pdf

Research Area: Neuro science; Immunity

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AEA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AEA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AEA in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: N-Arachidonoylethanolamine