Human Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
Human Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
Description
| Reference | ENT-E10477 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 4.48 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Lenght | 3.5h |
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Research Area: Infection immunity;Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: Amyloid Beta Peptide 1-40