Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Description
| Reference | ENT-E3316 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 1.45 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Lenght | 2.5h |
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Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: 25 Hydroxy Vitamin D;