Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Description
| Reference | ENT-E32767 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.1 ng/mL |
| Standard | 8 ng/mL |
| Detection Range | 0.13-8 ng/mL |
| Sample Type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 2h |
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Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 15-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 15-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 15-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: 15-HETE; 15-Hydroxyeicosatetraenoic Acid)