His-tag rabbit pAb
ENT-A1700
Description
| REF | ENT-A1700 |
|---|---|
| Category | Antibody Polyclonal |
| Description | His-tag rabbit pAb |
| Source | Rabbit |
| Applications | WB;ELISA |
| Reactivity | Species independent |
| Reactivity | Species independent |
| Dilution | Western Blot: 1/1000 – 1/3000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | 6X His synthetic peptide conjugated to KLH. |
| Storage Stability | -20°C/1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band KD | 15kD |
| Human Gene ID | |
| Human Swiss Prot Nº | |
| Subcellular Location |
Other Name: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Background: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.