Flag-Tag Mouse mAb
ENT-A1386
Description
| Ref | ENT-A1386 |
|---|---|
| Category | Antibody Monoclonal |
| Alternative Name | |
| Application | WB, IP, IF |
| Source | Mouse |
| Reactivity | |
| Dilution | WB 1:5,000 IP 1:200 IF 1:2,000 |
| Immunogen | Synthetic Peptide |
| Storage Stability | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Concentration | 1mg/mL |
| Observed Band(KD) | |
| Human Gene ID | |
| Human Swiss Prot No | |
| Subcellular Location |
Background: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.