EasyStep Human IL6(Interleukin 6) ELISA Kit
EasyStep Human IL6(Interleukin 6) ELISA Kit
Description
| Reference | ENT-E1183 |
|---|---|
| Category | Human A-E |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Standard | 100 pg/mL |
| Detection Range | 1.56-100 pg/mL |
| Sample Type | serum, plasma |
| Assay Lenght | 1.5 h |
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Research Area: Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6, and the Human IL6 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL6. After TMB substrate solution is added, only those wells that contain Human IL6 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor