EasyStep Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
EasyStep Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Description
| Reference | ENT-E3915 |
|---|---|
| Category | Human A-E |
| Size | 96T |
| Assay Type | Sandwich |
| Sensitivity | 37.66 pg/mL |
| Standard | 6000 pg/mL |
| Detection Range | 93.75-6000 pg/mL |
| Sample Type | Serum, plasma |
| Assay Lenght | 1.5h |
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha