EasyStep Human IGFBP1(Insulin Like Growth Factor Binding Protein 1) ELISA Kit
EasyStep Human IGFBP1(Insulin Like Growth Factor Binding Protein 1) ELISA Kit
Description
| Reference | ENT-E3665 |
|---|---|
| Category | Human A-E |
| Size | 48T |
| Assay Type | Sandwich |
| Sensitivity | 0.54 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | Serum, plasma |
| Assay Lenght | 1.5h |
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Research Area: Tumor immunity;Endocrinology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP1, and the Human IGFBP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP1. After TMB substrate solution is added, only those wells that contain Human IGFBP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein