Crk II (phospho Tyr221) rabbit pAb
ENT-A1954
Description
| REF | ENT-A1954 |
|---|---|
| Category | Antibody Polyclonal |
| Description | Crk II (phospho Tyr221) rabbit pAb |
| Source | Rabbit |
| Applications | WB;IF;ELISA |
| Reactivity | Human;Mouse;Rat;Monkey |
| Reactivity | Human;Mouse;Rat;Monkey |
| Dilution | Western Blot: 1/500 – 1/2000. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. AA range:187-236 |
| Storage Stability | -20°C/1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band KD | 40kD |
| Human Gene ID | 1398 |
| Human Swiss Prot Nº | P46108 |
| Subcellular Location | Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. . |
Other Name: CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38
Background: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],