AAK1 rabbit pAb
ENT-A2058
Description
| REF | ENT-A2058 |
|---|---|
| Category | Antibody Polyclonal |
| Description | AAK1 rabbit pAb |
| Source | Rabbit |
| Applications | WB;IHC;IF;ELISA |
| Reactivity | Human;Mouse;Rat;Monkey |
| Reactivity | Human;Mouse;Rat;Monkey |
| Dilution | Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. Immunofluorescence: 1/200 – 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AAK1. AA range:271-320 |
| Storage Stability | -20°C/1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band KD | 94kD |
| Human Gene ID | 22848 |
| Human Swiss Prot Nº | Q2M2I8 |
| Subcellular Location | Cell membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse, presynapse . Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells. . |
Other Name: AAK1; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1
Background: Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],