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Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit

Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit

Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit

Description

Reference ENT-E11476
Category Human F-N
Size 96T*5
Assay Type Competitive Inhibition
Sensitivity 0.94 ng/mL
Standard 200 ng/mL
Detection Range 3.13-200 ng/mL
Sample Type serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Lenght 2h

PDF File: 

Research Area: Metabolic pathway;Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NTXI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTXI in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: NTX-I; NTX1