Human IA-2 Ab(Insulinoma associated antigen 2 Autoantibody) ELISA Kit
Human IA-2 Ab(Insulinoma associated antigen 2 Autoantibody) ELISA Kit
Description
| Reference | ENT-E3841 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 26.8 U/mL |
| Standard | 4000 U/mL |
| Detection Range | 62.5-4000 U/mL |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IA-2 Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IA-2 Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IA-2 Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IA-2 Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: IA2 Ab