Human GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
Human GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
Description
| Reference | ENT-E5225 |
|---|---|
| Category | Human F-N |
| Size | 48T |
| Assay Type | Sandwich |
| Sensitivity | 5.8 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein; Neutrophil Activating Protein 3