Human FABP5(Fatty Acid Binding Protein 5, Epidermal) Microsample ELISA Kit
Human FABP5(Fatty Acid Binding Protein 5, Epidermal) Microsample ELISA Kit
Description
| Reference | ENT-E14380 |
|---|---|
| Category | Human F-N |
| Size | 96T*5 |
| Assay Type | Sandwich |
| Sensitivity | 0.124 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids |
| Assay Lenght | 3.5h |
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Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FABP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FABP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FABP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FABP5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: E-FABP; EFABP; PA-FABP; PAFABP; Psoriasis-Associated; Epidermal-type fatty acid-binding protein; Psoriasis-associated fatty acid-binding protein homolog