Human FA(Folic Acid) ELISA Kit
Human FA(Folic Acid) ELISA Kit
Description
| Reference | ENT-E34642 |
|---|---|
| Category | Human F-N |
| Size | 96T |
| Assay Type | Competitive Inhibition |
| Sensitivity | 15 pg/mL |
| Standard | 10000 pg/mL |
| Detection Range | 156.25-10000 pg/mL |
| Sample Type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Lenght | 2h |
PDF File: https://enteksci.com/wp-content/uploads/2025/10/ENT-E34642.pdf
Research Area: Hematology;Reproductive science;Nutrition metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human FA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names: VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate